Journal: Biosensors

Article Title: A Customized Microfluidic Paper-Based Platform for Colorimetric Immunosensing: Demonstrated via hCG Assay for Pregnancy Test

doi: 10.3390/bios11120474

Figure Lengend Snippet: ( A ) Schematic representation of hCG µPAD assay working principle: hCG analyte will bind to the anti-beta hCG antibody conjugated on AuNPs. The hCG−probe complex will move toward the T and C zones. The formed complex will be captured at the T zone by immobilized hCG alpha antibody. Free excess probe will travel further along the microfluidic channels until they reach the C zone, where they are captured by the anti-IgG antibody. ( B ) Actual photographs of hCG µPAD assay presenting both the positive and negative responses to hCG analyte.

Article Snippet: Mouse anti-beta hCG monoclonal antibody-colloid gold (AuNPs) conjugate was purchased from Abcam (Melbourne, Australia).

Techniques:

Journal: Biosensors

Article Title: Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

doi: 10.3390/bios9020068

Figure Lengend Snippet: ( a ) TEM image of the prepared AuNPs; ( b ) AuNPs size histogram distribution.

Article Snippet: X-ray photoelectron spectroscopy (XPS) analysis of casein-conjugated AuNPs was carried out using a K-Alpha XPS system (Thermo Fisher Scientific, Waltham, MA, USA) that is equipped with a microfocused monochromatic Al Ka X-ray source (1486.6 eV).

Techniques:

Journal: Biosensors

Article Title: Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

doi: 10.3390/bios9020068

Figure Lengend Snippet: ( a ) UV–visible spectra of bare AuNPs and the casein–AuNPs conjugates; ( b ) TEM image of the casein–AuNPs; ( c ) The whole XPS spectrum of casein@AuNPs; ( d ) XPS spectrum showing the two peaks of the Au4f spin–orbit doublet binding energy.

Article Snippet: X-ray photoelectron spectroscopy (XPS) analysis of casein-conjugated AuNPs was carried out using a K-Alpha XPS system (Thermo Fisher Scientific, Waltham, MA, USA) that is equipped with a microfocused monochromatic Al Ka X-ray source (1486.6 eV).

Techniques: Binding Assay

Journal: Biosensors

Article Title: Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

doi: 10.3390/bios9020068

Figure Lengend Snippet: ( a ) Cyclic voltammograms recorded from +1.35 to −1.40 V at a scan rate of 50 mV/s for a 1 M HCl solution and for increasing concentrations of AuNPs in 1 M HCl. ( b ) Chronoamperograms recorded by applying a potential of −1.00 V for 300 s, using a 1 M HCl solution and the same AuNPs concentrations in 1 M HCl.

Article Snippet: X-ray photoelectron spectroscopy (XPS) analysis of casein-conjugated AuNPs was carried out using a K-Alpha XPS system (Thermo Fisher Scientific, Waltham, MA, USA) that is equipped with a microfocused monochromatic Al Ka X-ray source (1486.6 eV).

Techniques:

Journal: American Journal of Cancer Research

Article Title: Enhanced proton treatment with a LDLR-ligand peptide-conjugated gold nanoparticles targeting the tumor microenvironment in an infiltrative brain tumor model

doi:

Figure Lengend Snippet: Radiosensitivity and ROS production in glioma cells treated in various ways. (A, B) F98 and U373 cells were exposed to proton beam for 48 h and/or pretreated bare AuNPs or ApoB@AuNPs 24 h prior to radiation for cell counting (A), the MTT assay (B). *P<0.05, **P<0.01, ***P<0.001. (C) Analysis of ROS generation in F98 and U373 cell lines 72 h after treatment with the indicated treatment by a ROS detection kit. *P<0.05, **P<0.01, ***P<0.001. (D) Analysis of cell death in F98 and U373 cell lines 72 h after treatment with the indicated treatment using a cell death detection kit. *P<0.05, **P<0.01, ***P<0.001. The highest sensitivity and ROS production were observed in ApoB@AuNP-treated glioma cells.

Article Snippet: ApoB@AuNPs or ApoB@AuNPs-Cy5s were synthesized by thiolating the ApoB peptide (3-mercaptopropionic acid-DWLKAFYDKVAEKLKEAFRLTRKRGLKLA-NH2: Anygen, Korea) or SH-PEG5000-Cy5.5 (Intechim, France) together with peptide to 15-nm gold NPs (Nanoprobe, USA) using the same method described previously [ 23 ].

Techniques: Cell Counting, MTT Assay

Journal: American Journal of Cancer Research

Article Title: Enhanced proton treatment with a LDLR-ligand peptide-conjugated gold nanoparticles targeting the tumor microenvironment in an infiltrative brain tumor model

doi:

Figure Lengend Snippet: Traversing of various molecular groups over the BBB model. LDLR-targeting ApoB@AuNPs showed enhanced transmittance compared with bare gold NPs in a dose-dependent manner. Baseline is a measured optical absorption of ApoB@AuNP that was assumed as transmitted through the membrane entirely for a given amounts of applied nanoparticles.

Article Snippet: ApoB@AuNPs or ApoB@AuNPs-Cy5s were synthesized by thiolating the ApoB peptide (3-mercaptopropionic acid-DWLKAFYDKVAEKLKEAFRLTRKRGLKLA-NH2: Anygen, Korea) or SH-PEG5000-Cy5.5 (Intechim, France) together with peptide to 15-nm gold NPs (Nanoprobe, USA) using the same method described previously [ 23 ].

Techniques:

Journal: American Journal of Cancer Research

Article Title: Enhanced proton treatment with a LDLR-ligand peptide-conjugated gold nanoparticles targeting the tumor microenvironment in an infiltrative brain tumor model

doi:

Figure Lengend Snippet: Fluorescence imaging of ex vivo brain haploids in which ApoB@AuNP (A, C) or bare gold NPs (B, D) were given intravenously prior to sacrifice and extraction. Observation of the red fluorescence of ApoB@AuNP-Cy5 in endothelial cell proliferation or perivascular tumor cell migration in the TME at 24 hours post intravenous injection of NPs (E). (F, G) Expanded view of (E) showed distribution of NPs-uptake at the neoplastic sites of TME more clearly.

Article Snippet: ApoB@AuNPs or ApoB@AuNPs-Cy5s were synthesized by thiolating the ApoB peptide (3-mercaptopropionic acid-DWLKAFYDKVAEKLKEAFRLTRKRGLKLA-NH2: Anygen, Korea) or SH-PEG5000-Cy5.5 (Intechim, France) together with peptide to 15-nm gold NPs (Nanoprobe, USA) using the same method described previously [ 23 ].

Techniques: Fluorescence, Imaging, Ex Vivo, Migration, Injection

Journal: Microorganisms

Article Title: Metal Nanoparticles to Combat Candida albicans Infections: An Update

doi: 10.3390/microorganisms11010138

Figure Lengend Snippet: Main mechanisms of action of metallic nanoparticles against C. albicans. Metal nanoparticles exhibit activity against C. albicans by metal ion release, adhesion, accumulation and damage to the cell wall and cell membrane, inhibition of electron-transport with consequent reduction in ATP levels, enzyme inactivation, protein oxidation and denaturation, increased production of reactive oxygen species (ROS), mitochondria dysfunction, and DNA damage.

Article Snippet: Yadav et al. [ ] designed tyrosol-functionalized chitosan gold nanoparticles (Chi-TY-AuNPs).

Techniques: Activity Assay, Membrane, Inhibition

Journal: Microorganisms

Article Title: Metal Nanoparticles to Combat Candida albicans Infections: An Update

doi: 10.3390/microorganisms11010138

Figure Lengend Snippet: Characteristics and main activities of metal nanoparticles against C. albicans included in the study.

Article Snippet: Yadav et al. [ ] designed tyrosol-functionalized chitosan gold nanoparticles (Chi-TY-AuNPs).

Techniques: Zeta Potential Analyzer, Inhibition, In Vitro, Activity Assay, In Vivo, Titanium Dioxide